Jiye Gu1,
Young Jin Park1,
Chaeyeong Ahn1,
Dayoung Heo1,
Yuseong Chung1,
Kang Min Kim2,
Jae Seon Kang1,
Jaesung Pyo1 
For correspondence:- Jaesung Pyo Email: jspyo@ks.ac.kr Tel:+82516634881
Accepted: 16 May 2021 Published: 30 June 2021
Citation: Gu J, Park YJ, Ahn C, Heo D, Chung Y, Kim KM, et al. Metabolic determination of decursinol using human liver microsome. Trop J Pharm Res 2021; 20(6):1165-1170 doi: 10.4314/tjpr.v20i6.10
© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine new metabolites of the main components of Angelica gigas known to give anti-inflammation and pain relief
Methods: Decursinol and blank sample were metabolized in human liver microsomes. The metabolized samples were centrifuged and deproteinated by adding 3 mL acetonitrile. The acetonitrile layer was concentrated and reconstituted in methanol. Finally, the prepared sample was injected into the LC-Q-TOF-MS.
Results: Four new metabolites of decursinol with m/z ranging from 263.0912 ~ 263.0920 were identified as hydroxylated forms of decursinol, and the hydroxylated position of each metabolite was characterized using TOF mass spectrum. Their error values of detected m/z were 0.38 ~ 2.29 ppm, which indicates high accuracy of analysis.
Conclusion: Previously unreported decursinol metabolites have been identified in this study. The findings provide athe basis for further pharmaceutical studies and functional food development using decursinol.??????
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